limiting dilution是什么意思_limiting dilution中文

limiting dilution中文什么意思

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Hybridomas secreting monoclonal antibodies ( mcabs ) specific for clostridium perfringens type a enterotoxin ( cpe ) were produced by fusion of nso myelomas cells with spleen cells from balb / c mice immunized with purified cpe . wells containing hybridomas secreting immunoglobulin g ( igg ) antibodies against cpe were specifically identified by an indirect enzyme - linked immunosorbent assay ( elisa ) , and two strains of elisa - positive hybridomas were selected and cloned forth by limited dilution
该纯化cpe对小白鼠的半数致死量为2 . 5ug /只；该cpe豚鼠皮肤蓝斑单位为2500 4000 ；引起新西兰白兔小肠积液的最小毒素量为25ug 。将纯化的cpe ，在含0 . 4福尔马林的的pb液中透析制备类毒素。

limiting dilution中文什么意思